How to Determine Levels of Vitamin C in the Fruits

Materials :

1.      Measure squash

2.      Drop pipette

3.      Measure pipette

4.      Buret and statif

5.      Erlenmeyer

6.      Funnel glass

7.      Beaker glass

8.      Filtering paper

9.      Suction ball

1.  Amilum solution 1%

1.  Iodium solution

1.  Orange fruit

Steps :

1.        Pelled rind (orange fruit).

2.        weigh the empty of beaker glass.

3.        Squeeze fruit in a beaker glass.

4.        Measure about twenty grams.

5.        Enter in a measure squash one hundred milliliters.

6.        Add distilled water to mark boundaries.

7.        Shake and filter using a filtering paper.

8.        Take twenty five milliliters of the filtrate.

9.        Pour solution in erlenmeyer.

10.    Add amilum solution 2 ml.

11.    Titrate with iodium solution.

12.    Account degree of vitamin C

How to Determine Levels of Vitamin C in the Fruits

In orange fruit contains nutrients including vitamins C. All vitamins are basically useless and good for the body. However, the efficacy of Vitamin C appears to have advantages.  Except as human imunity, vitamin C is also usefull for skin health. Vitamin C is also the most widely consumed vitamin person. Before you perform determine levels of vitamin C in the fruit you need measure squash, drop pipette, measure pipette, buret and statif, erlenmeyer, funnel glass, beaker glass, filtering paper, suction ball, amilum solution 1%, iodium solution and don’t forget need an orange fruit as sampel. There are steps that must be perform to determine levels of vitamin C. First, Peel the rind of orange fruit because all it takes is flesh. Second, weigh the empty of beaker glass using an analytical balance. After you known weight, squeeze this fruit and put in a beaker glass that has been weighed earlier. Third, weigh 25 mg of orange fruit that is contained in the beaker glass. To determine the weight of orange juice, with calculate of heavy beaker glass containing orange fruit reduce with an empty beaker glass, so you know the weight of 25 mg orange juice in accordance with procedures. After that, put the orange  juice  into a 100 ml measure squash and add distilled water to mark boundaries with drop pipette. Then the solution is shake and filter use filtering paper with funnel glass, so between sediment and filtrate can be separated. Then, take 25 ml of the filtrate in the form of the solution with use suction ball and measure pipette and insert the solution into erlenmeyer. In addition, add 2 ml of amilum solution as an indicator to easy observation of the color change when the titration. Furthermore, titrate with iodium solution until the solution changes color from clear to blue with use erlenmeyer. After that, calculate levels of vitamin C contain in orange fruits by the formula:

Levels =    x 100%

With this procedure, you can determine the levels of vitamin C in any fruit that contains vitamin C indicate. In short, that’s the procedures how to determine the levels of vitamin C in the fruits.